Sevilla's Jesus 'Tecatito' Corona will miss the World Cup after the Mexico international fractured his fibula in Thursday's training session.
The winger also suffered ligament damage in his left ankle.
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The LaLiga club announced Corona will undergo surgery on Thursday and is expected to be out of action between four and five months.
"He has suffered a major injury in training," Sevilla said in a statement.
"Tests have revealed that he suffers from a fractured fibula and deltoid ligament in his left ankle."
Corona, who joined Sevilla from FC Porto earlier this year, started in the Spanish side's league opener, a 2-1 loss at Osasuna on Aug. 12.
Sevilla coach Julen Lopetegui had feared Corona's injury was serious.
Speaking ahead of Friday's league clash against Almeira, Lopetegui said: "Tecatito got injured this morning.
"It doesn't look good. He was taken by ambulance and I hope it's not serious, but I insist, it doesn't look good."
Corona, 29, has scored 10 goals in 71 appearances for Mexico since making his national team debut in 2014.
